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A STUDY OF NI-RESISTANT BACTERIA ON THE RESTORED STAINLESS STEEL CROWN

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Abstract

kabstract :
¼Ò¾ÆÀÇ À¯±¸Ä¡ ¼öº¹¿¡ ÈçÈ÷ »ç¿ëÇÏ´Â stainless steel crownÀº ÁÖ¼ººÐÀ¸·Î 70¡­90%ÀÇ ´ÏÄÌ
°ú 5¡­15%ÀÇ Å©·ÒÀ» ÇÔÀ¯ÇÏ°í ÀÖ´Ù. ÀÌ Áß ´ÏÄÌ ÀÚü°¡ ÀÏÀ¸Å³ ¼ö ÀÖ´Â allergy¹ÝÀÀÀ̳ª ¹ß
¾Ï¼ºµîÀÇ ºÎÀÛ¿ëÀÌ ¼ºÀο¡¼­´Â È°¹ßÇÏ°Ô ¿¬±¸µÇ°í ÀÖÀ¸¸ç, ƯÈ÷ ´ÏÄÌÀÌ Æ÷ÇÔµÈ ÇÕ±ÝÀ» ÀÌ¿ë
ÇÑ º¸Ã¶¹° ÁÖº¯¿¡ º´¿ø¼ºÀÌ ÀÖ´Â ´ÏÄ̳»¼º Enterococci°¡ Á¸ÀçÇÑ´Ù´Â º¸°í°¡ ÀÖ¾î ¼ºÀΰú´Â
±¸°­È¯°æÀÌ ´Ù¸¥ ¼Ò¾Æ¿¡¼­µµ stainless steel crown ÁÖº¯¿¡ ´ÏÄ̳»¼º±ÕÁÖ°¡ Á¸ÀçÇÏ´ÂÁö¸¦ È®
ÀÎÇÏ°íÀÚ ÀÌ ½ÇÇèÀ» ½ÃÇàÇÏ¿´´Ù. À¯±¸Ä¡¿¡ stainless steel crown (Anatom primary crown,
Sankin)À» 6°³¿ù ÀÌ»ó ÀåÂøÇÑ È¯ÀÚ 15¸í°ú À¯±¸Ä¡ Çùº¯¿¡ Ä¡¾Æ¿ì½ÄÁõÀÌ ¾ø´Â ȯÀÚ 15¸íÀÇ
Ä¡Àº¿­±¸¾×¿¡¼­ äÃëÇÑ ½Ã·á¸¦ BHI ÇÑõ¹èÁö, ´ÏÄ̹èÁö ¹× Bile-esculin azide(BEA) ÇÑõ
¹èÁö¿¡ µµ¸»ÇÏ¿´´Ù. BEA ÇÑõ¹èÁö¿¡¼­ ÀÚ¶õ enterococci¸¦ ´ÏÄ̳»¼ºÁ¤µµ¸¦ ÇÐÀÎÇϱâ À§ÇØ
3, 5, 10, 30, 50 mM ´ÏÄ̹èÁö¿¡ Á¢Á¾ÇÑ ÈÄ ¼ºÀå»óŸ¦ °üÂûÇÏ¿´´Ù. ½ÇÇ豺¿¡¼­´Â BHI ÇÑõ
¹èÁö¿¡ 507, 350°³ÀÇ ±ÕÁÖ°¡ ºÐ¸®µÇ¾úÀ¸¸ç, ÀÌ Áß ´ÏÄ̳»¼º¼¼±Õ(3mM)Àº 53,864±ÕÁÖ¿´´Ù. ´ë
Á¶±º¿¡¼­´Â BHI ÇÑõ¹èÁö¿¡¼­ 414,590 ±ÕÁÖ°¡, ´ÏÄ̳»¼º¼¼±Õ(3mM)Àº 37,523 ±ÕÁÖ°¡ ºÐ¸®µÇ
¾ú´Ù. BEA ÇÑõ¹èÁö¿¡¼­´Â ½ÇÇ豺ÀÇ °æ¿ì 95°³°¡ ºÐ¸®µÇ¾úÀ¸¸ç, ´ëÁ¶±º¿¡¼­´Â 20°³ÀÇ ±ÕÁÖ
¸¸ÀÌ ºÐ¸®µÇ¾ú´Ù. BHI ÇÑõ¹èÁö¿¡¼­ ºÐ¸®µÈ ÃÑ ±ÕÁÖ ¼ö¸¦ 100À¸·Î ³õ¾ÒÀ» ¶§ 3mM ´ÏÄ̹è
Áö¿¡¼­ ºÐ¸®µÈ ±ÕÁÖ´Â ½ÇÇ豺¿¡¼­´Â 10.62%, ´ëÁ¶±º¿¡¼­´Â 9.05%¸¦ Â÷ÁöÇÏ¿© µÎ ±º°£¿¡ ¶Ñ
·ÇÇÑ Â÷ÀÌ´Â ¾ø¾úÀ¸¸ç, 3mM ´ÏÄ̹èÁö¿¡¼­ ÀÚ¶õ ÃÑ ±ÕÁÖ ¼ö¿Í BEA ÇÑõ¹èÁö¿¡¼­ ¹è¾çµÈ
enterococci¸¦ 3mM ´ÏÄ̹èÁö·Î ¿Å°Ü ¹è¾çÇßÀ» ¶§¿Í ºñ±³Çϸé, ½ÇÇ豺¿¡¼­ 0.13%, ´ëÁ¶±º¿¡
¼­ 0.03%·Î ¸ðµÎ 1%ÀÌÇÏÀÇ ºÐÆ÷¸¦ Â÷ÁöÇÏ¿´´Ù.
#ÃÊ·Ï#
-Abstract-
Stainless steel crowns are widely used for restoration for primary molars. The
material used for the crowns is an alloy of 70¡­80% nickel and 5¡­15% chromium.
Nickel has been known to cause allergic reaction, cancer and cell toxicity. Little is
known about nickel with respect to the relationship between Ni-contained SS crown and
gaining of Ni-resistance in oral microorganisms. The purpose of this study is to
examine whether use of Ni-contained SS crown leads to occurrence of Ni-resistant
microorganism, especially enterococci. The gingival crevicular fluid of two different
groups was taken. Experimental group included patients wearing SS crown, and control
group comprised individuals without SS crown. The samples were plated in BHI agar.
BHI agar supplemented with nickel chloride at the concentration of 3mM and bile
esculin azide (BEA) agar. The cultured enterococci on BEA agar medium were tested
their Ni-resistance in nickel-containing media increasing concentrations from 3mM to
50mM.
The results were as follow :
1. In experimental group, a total of 507,350 strains were isolated on BHI agar, of
which 53,864(10.62%) strains were found to be resistant to 3mM nickel. In control group.
of 414,590 isolates on BHI agar, 37,523 isolates were resistant to 3mM nickel.
2. A total of 95 enterococci were isolated on BEA agar in experimental group, while
20 were isolated in control group, of the enterococci, 68 and 12 isolates were found to
be nickel-resistant in experimental and control group, respectively.
3. Of 68 nickel-resistant isolates in experimental group, one survived 50mM nickel. In
contrast none of the isolates in control group was observed to grow at the
concentrations over 30mM nickel.

Å°¿öµå

±â¼º±Ý°ü; ´ÏÄ̳»¼º; enterococci; Stainless steel crown; Ni-resistant microorganism; Enterococci;

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